Any idea what caused this?

I did think that, but wondered why everything else on the same plain was in focus :thinking:

It's not on the same plane though, is it? Not on the plane of DoF which always runs parallel to the lens which is tilted down. The branch is higher, and closer to the lens.
 
It's not on the same plane though, is it? Not on the plane of DoF which always runs parallel to the lens which is tilted down. The branch is higher, and closer to the lens.

Focal plane, and could be tilted left or right and well as vertical, especially notice with macro and wide angle shots
 
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