As the previous post says, focus rails are rather tricky to use. It is difficult to get the camera on a rail on a tripod into the right position without disturbing the insect. Shooting a stack on a focus rail is also quite a slow process. The simplest alternative is to use "machine gun mode" to take a burst of photographs whilst you rock backwards and forwards. Just put it on the fastest multiple shot setting, compose, focus and then rock backwards and forwards a bit whilst holding the shutter down. It takes a lot of practice! The 90D also supports focus bracketting from the menus, so this can be used to take a burst of shots at a range of focus positions driven by the focus motor. Although, in my experience (using Magic Lantern on a 60D to achieve the same thing before Canon's firmware supported it), by the time you have set it all up - the subject has likely gone. For any of this to work, the subject has to stay completely still whilst you do all this stuff. Successfully capturing a stack of a live insect in the field is quite challenging, so best of luck!