Thanks Hoppy
I think I may be a "pixel peeper"
I also think I maybe trying to get a crystal picture of small birds with my 70-300 Canon IS USM. When possibly most of my shots are getting the subject only taking up 10/20% of the shot.
When I've posted "straight from camera" shots which are only cropped, I am being told to sharpen, then too much sharpening :bang:
When we talk cropping %, are we talking the amount of original frame you are removing? :shrug:
We're all pixel peepers! Just don't be too worried about what you see.
Everything looks woolly at 100%.
But I'm pretty sure your problem is cropping. Basically, don't. It is death to image quality. First rule for image quality is to
fill the frame with the subject, end of story. If you do that, and focus carefully (centre point, on the eyes) with a shake and blurr free shutter speed (that lens, IS on, 1/250sec or shorter) you will not be complaining about about sharpness or focus issues.
With small garden birds, that means a distance of less than 3m. If you can't get closer than that, you'll struggle. Birding is very difficult, it's one of the most testing subjects. You need very good technique, excellent field craft, and TBH, a massive lens is a big help.
On the focus micro-adjust thing, it's easy enough to check but you must be extremely careful, test a range of distances and focal lengths and if there's any change work out the best average. Just don't do it at a silly close distance or any corrections will throw the lens out at normal distance shooting range.
The closest we've got to an official guide to how it should be done is on this link form Chuck Westfall of Canon USA, second question down
http://www.digitaljournalist.org/issue0812/tech-tips.html He says minimum distance of 50x focal length but you'll find that's almost impossible to do in a meaningful way with your lens at 300mm. I would say don't go closer than 5m, which is not an unrealistic distance, but double-check infinity focus afterwards if you make any changes.